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| Zepeda,Andrea B.; Pessoa Jr,Adalberto; Farías,Jorge G.. |
| Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Methylotrophic yeast; Carbon source; Inducible promoter; Alcohol oxidase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500119 |
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| Arias,Cesar Andres Diaz; Marques,Daniela de Araujo Viana; Malpiedi,Luciana Pellegrini; Maranhão,Andrea Queiroz; Parra,Dulcineia Abdalla Saes; Converti,Attilio; Pessoa Junior,Adalberto. |
| Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor. Although in flasks the volumetric... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pichia pastoris; ScFv antibody fragment; Cryopreservation; Expression; Carbon source. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300419 |
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| XU,CHUNPING; YU,JINWEI; ZHAO,SHANSHAN; WU,SHUNGSHUNG; HE,PEIXIN; JIA,XUEWEI; LIU,YANQI; MAO,DUOBIN. |
| ABSTRACT The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phellinus vaninii Ljup; Exopolysaccharide; Carbon source; Antioxidant activity; Composition. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502033 |
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| Dantas,Adriana Cibele de Mesquita; Boneti,José Itamar; Nodari,Rubens Onofre; Guerra,Miguel Pedro. |
| The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malus; Marubakaido; M9; Culture medium; Carbon source. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600011 |
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| Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Droste,Annette. |
| Anathallis adenochila is a small epiphytic orchid, endemic to the Atlantic Forest in the southern and southeastern regions of Brazil. Information on the species is scarce and limited to distribution and occurrence data, with no reports about its development. In the present study, plantlets were propagated in vitro, and the influence of different concentrations of macronutrient salts and sucrose on the survival and development of the species was assessed. The analysis were performed on complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients) containing 10, 30 or 60 g L-1 of sucrose. Plantlet survival, height of the aerial part, and the length of the longest root were significantly greater on half MS medium containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Carbon source; Micropropagation; Orchid; Mineral salts. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400001 |
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| Shene,C; Leyton,A; Esparza,Y.; Flores,L; Quilodrán,B; Hinzpeter,I; Rubilar,M. |
| Thraustochytrids are marine protists found throughout the world in estuarine and marine habitats. These microorganisms have attracted interest, because their lipids contain different long chain polyunsaturated fatty acids (PUFAs). Those able to produce docosahexaenoic acid (C22:6 n-3, DHA) are the most studied because of the physiological importance of this PUFA in human beings. Their heterotrophic cultivation offers several challenges since biomass, lipid content, and fatty acid profile are dependent on growth conditions. In this work the effects of C source and its concentration on DHA production by different thraustochytrid strains are reviewed. Results obtained by different investigators on the use of alternative and low cost nutrient sources for... |
| Tipo: Journal article |
Palavras-chave: Thraustochytrium; Schizochytrium; Docosahexaenoic; Acid; Polyunsaturated fatty acids; Lipids; Carbon source; Nitrogen source. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100002 |
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| Furlan,Valcenir Júnior Mendes; Maus,Victor; Batista,Irineu; Bandarra,Narcisa Maria. |
| Abstract The high costs and environmental concerns associated with using marine resources as sources of oils rich in polyunsaturated fatty acids have prompted searches for alternative sources of such oils. Some microorganisms, among them members of the genus Aurantiochytrium, can synthesize large amounts of these biocompounds. However, various parameters that affect the polyunsaturated fatty acids production of these organisms, such as the carbon and nitrogen sources supplied during their cultivation, require further elucidation. The objective of this investigation was to study the effect of different concentrations of carbon and total nitrogen on the production of polyunsaturated fatty acids, particularly docosahexaenoic acid, by Aurantiochytrium sp. ATCC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carbon source; Docosahexaenoic acid; Nitrogen source; Polyunsaturated fatty acids; Thraustochytrids. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200359 |
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| Hu,Wei; Chen,Jihong; Wu,Qinghua; Li,Wenjian; Liu,Jing; Lu,Dong; Wang,Shuyang. |
| ABSTRACT Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L. thermophilus; Mutant; Heavy ion irradiation; L-(+)-lactic acid production; Carbon source; Fermentation characteristic. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100504 |
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